Journal: bioRxiv

Article Title: Temperature effects on taste preferences are influenced by TRPM8

doi: 10.64898/2026.02.18.706655

Figure Lengend Snippet: Warming taste fluids increases orosensory avoidance of static concentrations of quinine. TRPM8 deficient and B6 mice showed fewer licks to 0.03 ( A ) and 0.3 ( B ) mM quinine solutions proffered at 30° compared to 15°C ( p < 0.05). See methods section on Gardner-Altman plots for details on data presentation.

Article Snippet: These studies used adult female and male C57BL/6J (B6; strain 000664, The Jackson Laboratory [JAX], Bar Harbor, ME) and homozygous TRPM8 deficient/knockout (JAX strain 008198) mice.

Techniques:

Journal: bioRxiv

Article Title: Temperature effects on taste preferences are influenced by TRPM8

doi: 10.64898/2026.02.18.706655

Figure Lengend Snippet: Cooling and warming taste fluids interacts with stimulus concentration to affect orosensory responses to quinine. A , TRPM8 deficient and B6 mice showed increased licking towards high (0.3 mM) quinine when cool (15°C) compared to low (0.03 mM) quinine when warm (30°C), albeit this increase was small and not significant ( p > 0.05). Nonetheless, this temperature condition countered the ability of a higher quinine concentration to drive increased avoidance. B , In contrast, TRPM8 deficient and B6 mice showed markedly reduced licking to warm-high compared to cool-low quinine ( p < 0.05). See methods section on Gardner-Altman plots for details.

Article Snippet: These studies used adult female and male C57BL/6J (B6; strain 000664, The Jackson Laboratory [JAX], Bar Harbor, ME) and homozygous TRPM8 deficient/knockout (JAX strain 008198) mice.

Techniques: Concentration Assay

Journal: bioRxiv

Article Title: Temperature effects on taste preferences are influenced by TRPM8

doi: 10.64898/2026.02.18.706655

Figure Lengend Snippet: Concentration drives quinine avoidance at constant cool and warm fluid temperatures. TRPM8 deficient and B6 mice showed fewer licks to 0.3 compared to 0.03 mM quinine solutions when both were proffered at 15° ( A ) or 30°C ( B ) ( p < 0.05). See methods section on Gardner-Altman plots for details.

Article Snippet: These studies used adult female and male C57BL/6J (B6; strain 000664, The Jackson Laboratory [JAX], Bar Harbor, ME) and homozygous TRPM8 deficient/knockout (JAX strain 008198) mice.

Techniques: Concentration Assay

Journal: bioRxiv

Article Title: Temperature effects on taste preferences are influenced by TRPM8

doi: 10.64898/2026.02.18.706655

Figure Lengend Snippet: Effects of cooling and warming on orosensory responses to a low concentration of sugar. The influence of cool (15°C) and warm (30°C) taste fluid temperatures on licking to a low concentration (100 mM) of sucrose or glucose was not the same between mouse lines (interaction, p < 0.05), with warmth tending to increase and decrease responding in B6 control ( A ) and TRPM8 deficient ( B ) mice, respectively. See methods section on Gardner-Altman plots for details on presentation.

Article Snippet: These studies used adult female and male C57BL/6J (B6; strain 000664, The Jackson Laboratory [JAX], Bar Harbor, ME) and homozygous TRPM8 deficient/knockout (JAX strain 008198) mice.

Techniques: Concentration Assay, Control

Journal: bioRxiv

Article Title: Temperature effects on taste preferences are influenced by TRPM8

doi: 10.64898/2026.02.18.706655

Figure Lengend Snippet: Warming taste fluids causes orosensory avoidance of a high sugar concentration in male TRPM8 deficient mice. Female C57BL/6J control ( A ) and TRPM8 deficient ( B ) mice showed indifference to lick a high (500 mM) concentration of sucrose or glucose at cool (15°C) or warm (30°C) temperatures ( p > 0.05). In contrast, male C57BL/6J mice tended to increase their licks to sugars at 30° compared to 15°C ( C ) whereas TRPM8 deficient mice displayed significant avoidance of 30°C sugars ( D ) ( p < 0.05). See methods section on Gardner-Altman plots for details on presentation.

Article Snippet: These studies used adult female and male C57BL/6J (B6; strain 000664, The Jackson Laboratory [JAX], Bar Harbor, ME) and homozygous TRPM8 deficient/knockout (JAX strain 008198) mice.

Techniques: Concentration Assay, Control

Journal: bioRxiv

Article Title: Temperature effects on taste preferences are influenced by TRPM8

doi: 10.64898/2026.02.18.706655

Figure Lengend Snippet: TRPM8 deficient mice show reduced preference for high concentrations of sugars presented cool. Both C57BL/6J ( A ) and TRPM8 deficient ( B ) mice showed increased licking to a high concentration (500 mM) of sucrose or glucose presented cool (15°C) compared to a low concentration (100 mM) offered warm (30°C) ( p < 0.05). However, the increase in responding to cool-high sugars was markedly reduced in TRPM8 deficient mice. See methods section for details on Gardner-Altman plots.

Article Snippet: These studies used adult female and male C57BL/6J (B6; strain 000664, The Jackson Laboratory [JAX], Bar Harbor, ME) and homozygous TRPM8 deficient/knockout (JAX strain 008198) mice.

Techniques: Concentration Assay

Journal: bioRxiv

Article Title: Temperature effects on taste preferences are influenced by TRPM8

doi: 10.64898/2026.02.18.706655

Figure Lengend Snippet: Warning impairs preference for high sugars in TRPM8 deficient mice. A , C57BL/6J mice prefer to lick warm (30°C) high (500 mM) sugars over cool (15°C) low (100 mM) ( p < 0.05). In contrast, TRPM8 deficient mice do not significantly increase their licks to warm-high sugars ( p > 0.05). See methods for Gardner-Altman plot details.

Article Snippet: These studies used adult female and male C57BL/6J (B6; strain 000664, The Jackson Laboratory [JAX], Bar Harbor, ME) and homozygous TRPM8 deficient/knockout (JAX strain 008198) mice.

Techniques:

Journal: bioRxiv

Article Title: Temperature effects on taste preferences are influenced by TRPM8

doi: 10.64898/2026.02.18.706655

Figure Lengend Snippet: TRPM8 deficient mice show reduced sugar preference at constant taste fluid temperatures. C57BL/6J ( A ) and TRPM8 deficient ( B ) mice significantly increased their licking to a high concentration (500 mM) of sucrose or glucose compared to low (100 mM) when both solution temperatures were held constant at 15° or 30°C during tests ( p < 0.05). This effect did not differ between temperatures ( p > 0.05) and plotted data are collapsed across them. The observed increase in licking to high sugars was reduced in TRPM8 deficient mice. See methods section on Gardner-Altman plots for details on data presentation.

Article Snippet: These studies used adult female and male C57BL/6J (B6; strain 000664, The Jackson Laboratory [JAX], Bar Harbor, ME) and homozygous TRPM8 deficient/knockout (JAX strain 008198) mice.

Techniques: Concentration Assay

Journal: bioRxiv

Article Title: Temperature effects on taste preferences are influenced by TRPM8

doi: 10.64898/2026.02.18.706655

Figure Lengend Snippet: Warming taste fluids increases orosensory avoidance of static concentrations of quinine. TRPM8 deficient and B6 mice showed fewer licks to 0.03 ( A ) and 0.3 ( B ) mM quinine solutions proffered at 30° compared to 15°C ( p < 0.05). See methods section on Gardner-Altman plots for details on data presentation.

Article Snippet: TRPM8 deficient mice are derived from backcrosses with C57BL/6 mice (JAX).

Techniques:

Journal: bioRxiv

Article Title: Temperature effects on taste preferences are influenced by TRPM8

doi: 10.64898/2026.02.18.706655

Figure Lengend Snippet: Cooling and warming taste fluids interacts with stimulus concentration to affect orosensory responses to quinine. A , TRPM8 deficient and B6 mice showed increased licking towards high (0.3 mM) quinine when cool (15°C) compared to low (0.03 mM) quinine when warm (30°C), albeit this increase was small and not significant ( p > 0.05). Nonetheless, this temperature condition countered the ability of a higher quinine concentration to drive increased avoidance. B , In contrast, TRPM8 deficient and B6 mice showed markedly reduced licking to warm-high compared to cool-low quinine ( p < 0.05). See methods section on Gardner-Altman plots for details.

Article Snippet: TRPM8 deficient mice are derived from backcrosses with C57BL/6 mice (JAX).

Techniques: Concentration Assay

Journal: bioRxiv

Article Title: Temperature effects on taste preferences are influenced by TRPM8

doi: 10.64898/2026.02.18.706655

Figure Lengend Snippet: Concentration drives quinine avoidance at constant cool and warm fluid temperatures. TRPM8 deficient and B6 mice showed fewer licks to 0.3 compared to 0.03 mM quinine solutions when both were proffered at 15° ( A ) or 30°C ( B ) ( p < 0.05). See methods section on Gardner-Altman plots for details.

Article Snippet: TRPM8 deficient mice are derived from backcrosses with C57BL/6 mice (JAX).

Techniques: Concentration Assay

Journal: bioRxiv

Article Title: Temperature effects on taste preferences are influenced by TRPM8

doi: 10.64898/2026.02.18.706655

Figure Lengend Snippet: Effects of cooling and warming on orosensory responses to a low concentration of sugar. The influence of cool (15°C) and warm (30°C) taste fluid temperatures on licking to a low concentration (100 mM) of sucrose or glucose was not the same between mouse lines (interaction, p < 0.05), with warmth tending to increase and decrease responding in B6 control ( A ) and TRPM8 deficient ( B ) mice, respectively. See methods section on Gardner-Altman plots for details on presentation.

Article Snippet: TRPM8 deficient mice are derived from backcrosses with C57BL/6 mice (JAX).

Techniques: Concentration Assay, Control

Journal: bioRxiv

Article Title: Temperature effects on taste preferences are influenced by TRPM8

doi: 10.64898/2026.02.18.706655

Figure Lengend Snippet: Warming taste fluids causes orosensory avoidance of a high sugar concentration in male TRPM8 deficient mice. Female C57BL/6J control ( A ) and TRPM8 deficient ( B ) mice showed indifference to lick a high (500 mM) concentration of sucrose or glucose at cool (15°C) or warm (30°C) temperatures ( p > 0.05). In contrast, male C57BL/6J mice tended to increase their licks to sugars at 30° compared to 15°C ( C ) whereas TRPM8 deficient mice displayed significant avoidance of 30°C sugars ( D ) ( p < 0.05). See methods section on Gardner-Altman plots for details on presentation.

Article Snippet: TRPM8 deficient mice are derived from backcrosses with C57BL/6 mice (JAX).

Techniques: Concentration Assay, Control

Journal: bioRxiv

Article Title: Temperature effects on taste preferences are influenced by TRPM8

doi: 10.64898/2026.02.18.706655

Figure Lengend Snippet: TRPM8 deficient mice show reduced preference for high concentrations of sugars presented cool. Both C57BL/6J ( A ) and TRPM8 deficient ( B ) mice showed increased licking to a high concentration (500 mM) of sucrose or glucose presented cool (15°C) compared to a low concentration (100 mM) offered warm (30°C) ( p < 0.05). However, the increase in responding to cool-high sugars was markedly reduced in TRPM8 deficient mice. See methods section for details on Gardner-Altman plots.

Article Snippet: TRPM8 deficient mice are derived from backcrosses with C57BL/6 mice (JAX).

Techniques: Concentration Assay

Journal: bioRxiv

Article Title: Temperature effects on taste preferences are influenced by TRPM8

doi: 10.64898/2026.02.18.706655

Figure Lengend Snippet: Warning impairs preference for high sugars in TRPM8 deficient mice. A , C57BL/6J mice prefer to lick warm (30°C) high (500 mM) sugars over cool (15°C) low (100 mM) ( p < 0.05). In contrast, TRPM8 deficient mice do not significantly increase their licks to warm-high sugars ( p > 0.05). See methods for Gardner-Altman plot details.

Article Snippet: TRPM8 deficient mice are derived from backcrosses with C57BL/6 mice (JAX).

Techniques:

Journal: bioRxiv

Article Title: Temperature effects on taste preferences are influenced by TRPM8

doi: 10.64898/2026.02.18.706655

Figure Lengend Snippet: TRPM8 deficient mice show reduced sugar preference at constant taste fluid temperatures. C57BL/6J ( A ) and TRPM8 deficient ( B ) mice significantly increased their licking to a high concentration (500 mM) of sucrose or glucose compared to low (100 mM) when both solution temperatures were held constant at 15° or 30°C during tests ( p < 0.05). This effect did not differ between temperatures ( p > 0.05) and plotted data are collapsed across them. The observed increase in licking to high sugars was reduced in TRPM8 deficient mice. See methods section on Gardner-Altman plots for details on data presentation.

Article Snippet: TRPM8 deficient mice are derived from backcrosses with C57BL/6 mice (JAX).

Techniques: Concentration Assay