Journal: Communications Chemistry

Article Title: Pharmacological tools to modulate ordered membrane domains and order-dependent protein function

doi: 10.1038/s42004-025-01874-8

Figure Lengend Snippet: a Average current traces of TRPM8 menthol response before and after exposure to the compounds in HEK293T cells expressing human TRPM8 from automated patch clamp experiments. Each dot is when the pulse program is applied. 100 μM menthol was applied for 75 s 4 times before perfusing 0.03% DMSO (control) or 10 µM compounds for 15 min. 100 μM menthol was then applied with corresponding compounds twice for 75 s. One n is the sum of 20 cells from an amplifier on the plate. b Average ratio of menthol response from each compound in TRPM8 HEK293T cells. The ratio calculated from data in ( a ), where the last two menthol responses before compound application were averaged and compared to the two menthol responses after compound application. P -values were determined by ANOVA followed by Dunnett’s tests. Bars are means ± SD. c Quantification of western blot analysis of phospho-EGFR (left) and ERK (right) from HeLa cells treated with compounds for 15 min then stimulated with EGF for 1 min. n = 3, bars are means ± SD. All comparisons are not significant.

Article Snippet: Cells were then labeled with either of two TRPM8 primary antibodies targeted to an extracellular epitope (Alomone, Cat. No. ACC-049 Abcepta, Cat. No. AP8181D).

Techniques: Expressing, Patch Clamp, Control, Western Blot

Journal: Current Research in Food Science

Article Title: Cooling sensation biosensor coupled with the Chou-Talalay method for quantitative assessment of interaction patterns in binary cooling agent mixtures

doi: 10.1016/j.crfs.2025.101287

Figure Lengend Snippet: Expression and functional characterization of HEK293-TRPM8 cells. (A) Immunofluorescence staining of TRPM8 in HEK293 cells and HEK293-TRPM8 cells. Scale bar = 50 μm. (B) Western blot analysis of TRPM8 protein expression in HEK293 cells and HEK293-TRPM8 cells. (C) Dose-dependent current responses of TRPM8 channels to various concentrations of menthol (10–1000 μM) in HEK293-TRPM8 cells. (D) Representative current obtained from the voltage-step protocol. HEK293-TRPM8 cells were treated by saline control and 1000 μM menthol across a voltage range of −100 mV to +240 mV. (E) Current density-voltage curves of HEK293-TRPM8 cells. the relationship between current density and applied voltage for TRPM8 channels in the presence of saline control and 1000 μM menthol. Each point represents the mean ± standard deviation (n = 10), ∗ p < 0.05.

Article Snippet: The following antibodies were used: TRPM8 (Proteintech, 12813-1-AP, 1:1000), GAPDH (Proteintech, 60004–1, 1:2000), Goat Anti-Mouse IgG-HRP (Abcam, AB6789, 1:2000), Goat Anti-Rabbit IgG-HRP (Abcam, AB6721, 1:2000).

Techniques: Expressing, Functional Assay, Immunofluorescence, Staining, Western Blot, Saline, Control, Standard Deviation

Journal: Current Research in Food Science

Article Title: Cooling sensation biosensor coupled with the Chou-Talalay method for quantitative assessment of interaction patterns in binary cooling agent mixtures

doi: 10.1016/j.crfs.2025.101287

Figure Lengend Snippet: Construction and evaluation of the cooling sensation biosensor. (A) The chemical structures of menthol, WS-3 and WS-23. (B) Representative real-time intracellular Ca 2+ responses of HEK293-TRPM8 cells to various concentrations of cooling agents. (C) Dose-response curves of menthol, WS-3 and WS-23 on the cooling sensation biosensor. Each point represents the mean ± standard deviation (n = 3). ∗: p < 0.05; ∗∗: p < 0.01; ∗∗∗: p < 0.001.

Article Snippet: The following antibodies were used: TRPM8 (Proteintech, 12813-1-AP, 1:1000), GAPDH (Proteintech, 60004–1, 1:2000), Goat Anti-Mouse IgG-HRP (Abcam, AB6789, 1:2000), Goat Anti-Rabbit IgG-HRP (Abcam, AB6721, 1:2000).

Techniques: Standard Deviation